Step-by-Step Guide to Safe Aplisol Tuberculin Skin Test Administration
Administering a tuberculin skin test is a crucial preventative health measure for detecting latent tuberculosis infections. Understanding the steps required for a safe and effective test is vital for healthcare professionals. This guide will focus on how to administer the Aplisol Tuberculin Skin Test, as well as its distinctions from the Tubersol Skin Tests.
Understanding Tuberculin Skin Testing
Tuberculin skin tests are diagnostic tools used to identify potential exposure to Mycobacterium tuberculosis. The test involves the intradermal injection of a purified protein derivative (PPD) and assessing the skin reaction. Two common substances used in these tests are Aplisol and Tubersol, each with specific properties and methods of administration.
Aplisol Tuberculin Skin Test
Aplisol is a form of PPD used to detect latent tuberculosis infections. This guide will outline the safe administration of the Aplisol Tuberculin PPD Purified Protein Derivative, focusing on the 5 TU /0.1 mL 5 mL solution (50 Tests), which you can find on Mountainside Medical.
Step-by-Step Guide: How to Administer the Aplisol Tuberculin Skin Test
Prepare the Patient
- Ensure the patient understands the procedure and purpose of the test.
- Obtain verbal consent and assess any contraindications or allergies.
- Cleanse the patient's forearm with an alcohol wipe and allow it to dry.
Select the Injection Site
Choose the inner surface of the forearm, approximately 2-4 inches below the elbow.
Draw the Solution
- Use a 1 mL tuberculin syringe with a 27-gauge needle.
- Withdraw 0.1 mL of Aplisol solution from the vial.
Inject the Test
- Stretch the skin at the chosen area.
- Insert the needle at a 5-15 degree angle with the bevel facing upwards.
- Slowly inject the solution, creating a small bleb (wheel) of about 6-10 mm in diameter.
Post-Injection Care
- Instruct the patient not to press or cover the injection site.
- Schedule a follow-up to read the results in 48-72 hours.
Read the Result
- Measure the induration (firm bump) in millimeters, not the redness.
- Consult guidelines to interpret the results based on the patient’s risk factors.
Comparison with Tubersol Skin Tests
While Aplisol and Tubersol are both used in tuberculin skin testing, they are different formulations of PPD and may have slight variations in sensitivity.
- Tubersol Tuberculin Purified Protein Derivative (Mantoux) 5 mL (50 Tests) is a comparable solution for testing. It can be explored further on Mountainside Medical.
- The Tubersol Tuberculin Purified Protein Derivative (Mantoux) 1 mL (10 Tests) Tween Stabilized Solution is another option available for specific needs. More information can be found here.
Safety and Handling Tips
- Storage: Keep Aplisol and Tubersol refrigerated to maintain their potency.
- Expiration: Check the expiration date before use to ensure effectiveness.
- Adverse Reactions: Monitor the patient for any adverse reactions post-injection, especially those with known hypersensitivities.
Conclusion
Understanding how to administer the Aplisol Tuberculin Skin Test is critical for healthcare professionals aiming to identify latent TB infections effectively. By following the outlined steps and safety protocols, practitioners can ensure accurate results and patient safety. For high-quality testing materials, you can explore options on Mountainside Medical.
Ensure to stay informed about the latest guidelines and updates in test administration to continue providing the best care for your patients.

